Dear Rachel, You don’t have any spots with a resolution lower than ca. 15Å, which means that none of the cell axes can be longer than 15Å. This must be a crystal of some small molecule and not of a protein and/or RNA molecule.
One question: you mention that the data could not be processed by HKL2000; where did then the unit cell you quote come from? You could try indexing your data with small-molecule settings, e.g. allowing a very small unit cell. Best, Herman Von: CCP4 bulletin board [mailto:[email protected]] Im Auftrag von Liu Rachel Gesendet: Donnerstag, 15. Dezember 2016 18:54 An: [email protected] Betreff: [ccp4bb] Could this be a complex crystal? or only RNA crystal? or micromolecular？ Dear everyone: Recently, I suffered a problem during my research work. I co-purified a zinc finger protein(152aa) and a dsRNA(19bp), the final SEC buffer is 20mM Hepes, 150mM NaCl, pH8.0. Then the complex was co-crystallized by vapor diffusion against a solution of 30% PEG400, 0.2M MgCl, 0.1M Tris pH8.4. The crystal is very beautiful, but the X-ray diffraction diagram is very strange, the diffraction point looks big and sparse(as shown in the picture). The Data cannot be processed with HKL2000 either. unit_cell = 24.808 42.863 119.042 90 90 90 space_group = "I 2 2 2" I wanna figure out, could this be a complex crystal? or only RNA crystal? or other micromolecular? PS. I've set drops without the protein in the sample, but prepare the sample of RNA with the protein buffer as if the protein was there. And there was no crystallization . Thank you very much! Rachel Liu Room 2071, research center in life sciences, China Agricultural University No. 2 yuanmingyuan west road, Haidian District, Beijing, 100193 P.R. China Tel: (86)-10-62734078
