dear Rachel,
to me this seems more like a salt diffraction
pattern... I agree with Kevin, run a SDS on your crystal and see if you
have crystallized something "interesting"
Good luck
On Fri, Dec 16, 2016 at 9:26 AM, Camillo Rosano <[email protected]>
wrote:
> dear Rachel,
> to me this seems more like a salt diffraction
> pattern... I agree with Kevin, run a SDS on your crystal and see if you
> have crystallized something "interesting"
> Good luck
> Camillo
>
> On Fri, Dec 16, 2016 at 9:10 AM, <[email protected]> wrote:
>
>> Dear Rachel,
>>
>>
>>
>> You don’t have any spots with a resolution lower than ca. 15Å, which
>> means that none of the cell axes can be longer than 15Å. This must be a
>> crystal of some small molecule and not of a protein and/or RNA molecule.
>>
>>
>>
>> One question: you mention that the data could not be processed by
>> HKL2000; where did then the unit cell you quote come from? You could try
>> indexing your data with small-molecule settings, e.g. allowing a very small
>> unit cell.
>>
>>
>>
>> Best,
>>
>> Herman
>>
>>
>>
>> *Von:* CCP4 bulletin board [mailto:[email protected]] *Im Auftrag
>> von *Liu Rachel
>> *Gesendet:* Donnerstag, 15. Dezember 2016 18:54
>> *An:* [email protected]
>> *Betreff:* [ccp4bb] Could this be a complex crystal? or only RNA
>> crystal? or micromolecular？
>>
>>
>>
>>
>>
>> Dear everyone:
>>
>>
>>
>> Recently, I suffered a problem during my research work. I
>> co-purified a zinc finger protein(152aa) and a dsRNA(19bp), the
>> final SEC buffer is *20mM Hepes, 150mM NaCl, pH8.0*. Then the
>> complex was co-crystallized by vapor diffusion against a solution of *30%
>> PEG400, 0.2M
>> MgCl, 0.1M Tris pH8.4. * The crystal is very beautiful, but the
>> X-ray diffraction diagram is very strange, the diffraction point looks
>> big and sparse(as shown in the picture). The Data cannot
>> be processed with HKL2000 either.
>>
>> unit_cell = 24.808 42.863 119.042 90 90 90
>>
>> space_group = "I 2 2 2"
>>
>>
>>
>> I wanna figure out, could this be a complex crystal? or only RNA
>> crystal? or other micromolecular?
>>
>>
>>
>> PS. I've set drops without the protein in the sample, but prepare
>> the sample of RNA with the protein buffer as if the protein was there. And
>> there was no crystallization .
>>
>>
>>
>> Thank you very much!
>>
>>
>>
>>
>>
>>
>>
>>
>>
>> Rachel Liu
>>
>> Room 2071, research center in life sciences,
>>
>> China Agricultural University
>>
>> No. 2 yuanmingyuan west road, Haidian District, Beijing, 100193 P.R. China
>>
>> Tel: (86)-10-62734078
>>
>>
>
