[ccp4bb] How can I use a reference model in Phenix refinement?

[Peng]

Dear all,

I met a problem when I tried to refine a EM structure using a reference model 
in Phenix.

I used the command

phenix.real_space_refine 1.pdb 2.mrc reference.eff  resolution=3.2 

, but it did not work. 




My reference.eff  is following:

refinement {

  reference_model {

    enabled = True

    reference_group {

      reference = chain A 

      selection = chain A

      file_name = 20161215-3.pdb

    }

    reference_group {

      reference = chain B

      selection = chain B

      file_name = 20161215-3.pdb

    }

    reference_group {

      reference = chain C

      selection = chain C




      file_name = 20161215-3.pdb

   }

   }

  }

}




My output .log file is following:

   reference_model {

      enabled = False

      file = None

      use_starting_model_as_reference = False

      sigma = 1.0

      limit = 15.0

      hydrogens = False

      main_chain = True

      side_chain = True

      fix_outliers = True

      strict_rotamer_matching = False

      auto_shutoff_for_ncs = False

      secondary_structure_only = False

      reference_group {

        reference = None

        selection = None

        file_name = None

......




It seemed my reference.eff was not recognized.

How can I use the reference model in Phenix refinement? 




Thanks,

Peng