Dear Amit,
Maybe try adding an equal volume of 8M urea to your sample before
adding the SDS-PAGE sample buffer. Then I'd test boiling and not boiling
that sample prior to loading on the gel.
Good luck,
Mark
On 21 February 2017 at 17:22, amit gaur <[email protected]> wrote:
> Hi all,
> I am trying to purify a potassium ion channel from insect cell using
> baculovirus expression system. I am not seeing monomer of this protein in
> SDS instead a dimer appears.So,I increased DTT in SDS buffer but no change
> and dimer was intact. In size exclusion this protein appeared as a tetramer
> which is common oligomerizaton of potassium channel family with GYG motif.
> Can any body suggest what should I do in this case?
>
> Thanks and regards,
>
>
> --
> *Dr. Amit Gaur*
>
>
> *Post Doctoral ResearcherPI: Dr. Ji-Fang ZhangThomas Jefferson University*
> *1020, Locust Street, Suite 418*
> *Philadelphia, PA 19107*
>
>
>
