Hi all,
I am trying to purify a potassium ion channel from insect cell using
baculovirus expression system. I am not seeing monomer of this protein in
SDS instead a dimer appears.So,I increased DTT in SDS buffer but no change
and dimer was intact. In size exclusion this protein appeared as a tetramer
which is common oligomerizaton of potassium channel family with GYG motif.
Can any body suggest what should I do in this case?
Thanks and regards,
--
*Dr. Amit Gaur*
*Post Doctoral ResearcherPI: Dr. Ji-Fang ZhangThomas Jefferson University*
*1020, Locust Street, Suite 418*
*Philadelphia, PA 19107*
