Darn it, you're right. JPK
-----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Hughes, Jon Sent: Thursday, March 30, 2017 4:05 AM To: [email protected] Subject: [ccp4bb] AW: [ccp4bb] [ccp4bb] protein precipitation reg yes, "oil of vitriol" is sulphuric acid, "blue vitriol" is copper II sulphate as i recall. j -----Ursprüngliche Nachricht----- Von: CCP4 bulletin board [mailto:[email protected]] Im Auftrag von CRAIG A BINGMAN Gesendet: Donnerstag, 30. März 2017 05:52 An: [email protected] Betreff: Re: [ccp4bb] [ccp4bb] protein precipitation reg Since I’m in full-on cranky old biochemist mode now, I think that vitriol is an old name for sulfuric acid. > On Mar 29, 2017, at 8:40 PM, Keller, Jacob <[email protected]> wrote: > >> And if we are going to pour scorn and vitriol on Tris, why not mention its >> large dpKa/dT of 0.03 pH units/deg ? > > Hah! That's what many people are doing when they make buffers: pouring > vitriol (HCl) on TRIS! I prefer to pour concentrated HEPES, and get two > buffers without adding any extra Cl-. > > JPK
