Hi Gaoyina, I guess your crystals ended up in slightly different lattices or with slightly different ordering during flash-cooling. I this case can see two possible solutions: - collect more complete data from a single crystal, you may have to "sacrifice" some resolution by collecting shorter images or attenuating the beam somewhat. - try to flash-cool the crystals in a more reproducible way, so they are more similar to each other. Another possibility might be that you have too many spot overlaps, in this case you may need to collect thinner-sliced images, offset the detector, put the detector further away (i.e. lower resolution), or measure a crystal with a different orientation (try put the spindle rotation more or less around the long cell axis). In any of these cases I think you will need to collect more data. With the current data, in Mosflm data processing you may be able to get more complete data with the SEPARATION CLOSE option, if you did not invoke this already. In other data processing programs there surely are similar options. On the other hand, in the automated data processing pipelines that many synchrotrons use now they are probably already activated when necessary (at least I have rarely been able to improve on automatically processed data when doing it myself later...)
Greetings, Mark J van Raaij Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://wwwuser.cnb.csic.es/~mjvanraaij > On 11 Apr 2017, at 04:46, 高艺娜 <gaoy...@cau.edu.cn> wrote: > > Dear all, > For my crystal, I have to merge 3-4 sets of diffraction data using HKL2000 > program to meet requirements for data completeness, but the problem is the > Rmerge value was too high to go on every time. > Did anyone have met the same problem and how to solve it? or some tips for > solve this problem？ > > All comments will be appreciated! > > Best Regards, >
