Minimizing a red bar may be tricky.. Have you tried to make it less red (blue or may be green)? Otherwise Rw/Rf~20/25 is just fine at 2.2A resolution. What exactly your worry is about? Pavel
On Mon, Apr 24, 2017 at 12:48 AM, Vipul Panchal <[email protected]> wrote: > Hi all, > > I am solving structure of one of the acyltransferse protein. We have > collected data at 2.16A resolution. Currently the Rfree is 0.2508 and > Rwork is 0.2042. > > *My query is regarding NCS difference.* Under validation tool of coot > while looking for NCS differene, i can find some residues with red bar. *Can > some one suggest me how may i minimize it if i am expected to do it?* > > > -- > Vipul Panchal > Senior Research Fellow, > Respiratory disease and biology, > CSIR-IGIB > (M)-9540113372 >
