I am not from any structure biology related group. So I have followed literatures​ for the structure solution and analysis. At this level all stereochemical and geometry looks good. The only parameter I could see with some red bar was NCS difference. *Through literature, I couldn't understand if some red bars in NCS DIFFERENCE window should be a concern.* So it would be helpful if some of you can suggest me some relevant literature or practice regarding NCS difference.
On 24-Apr-2017 1:25 PM, "Pavel Afonine" <[email protected]> wrote: Minimizing a red bar may be tricky.. Have you tried to make it less red (blue or may be green)? Otherwise Rw/Rf~20/25 is just fine at 2.2A resolution. What exactly your worry is about? Pavel On Mon, Apr 24, 2017 at 12:48 AM, Vipul Panchal <[email protected]> wrote: Hi all, I am solving structure of one of the acyltransferse protein. We have collected data at 2.16A resolution. Currently the Rfree is 0.2508 and Rwork is 0.2042. *My query is regarding NCS difference.* Under validation tool of coot while looking for NCS differene, i can find some residues with red bar. *Can some one suggest me how may i minimize it if i am expected to do it?* -- Vipul Panchal Senior Research Fellow, Respiratory disease and biology, CSIR-IGIB (M)-9540113372 On 24-Apr-2017 1:25 PM, "Pavel Afonine" <[email protected]> wrote: > Minimizing a red bar may be tricky.. Have you tried to make it less red > (blue or may be green)? Otherwise Rw/Rf~20/25 is just fine at 2.2A > resolution. What exactly your worry is about? > Pavel > > On Mon, Apr 24, 2017 at 12:48 AM, Vipul Panchal <[email protected]> > wrote: > >> Hi all, >> >> I am solving structure of one of the acyltransferse protein. We have >> collected data at 2.16A resolution. Currently the Rfree is 0.2508 and >> Rwork is 0.2042. >> >> *My query is regarding NCS difference.* Under validation tool of coot >> while looking for NCS differene, i can find some residues with red bar. *Can >> some one suggest me how may i minimize it if i am expected to do it?* >> >> >> -- >> Vipul Panchal >> Senior Research Fellow, >> Respiratory disease and biology, >> CSIR-IGIB >> (M)-9540113372 >> > >
