On 06/07/2017 10:46 AM, Bonsor, Daniel wrote:
It will either be two things. DNA or residual Triton-X-100.
Actually Triton X-100 absorbs more at 280 than 260 - in fact the hydroxyphenyl in TX-100 looks remarkably like tyrosine in RNase A. I long ago gave up hope of resolving triton from protein and DNA by spectral curve-fitting! eab
