Dear colleagues,
I am really astonished by a recently released structure 5XQL. This structure was just published in BBRC with a super fast process (accepted overnight). Significant inconsistences were observed when I compared the Data collection and refinement statistics in the paper and at PDB website. The paper shows the crystal belong to I 4 2 2 with a=135.77, b=136.11, c=95.13 (Å) ; α=90.01. The resolution is 2.5Å with completeness of 99.7 (99.1). However when I checked the released structure I got a= b=135.94, c=95.13 (Å) ; α=90.0. The resolution is 2.5Å with completeness of 81.5 (44.0). The completeness of the highest resolution shell is so low that 2.5Å seems unreasonable. At first I thought that the authors could be rookies in structural biology. But the molecular replacement in this paper could be very difficult due to very low sequence identity between the search model and the final structure. It was unlikely that two new hands could solve it easily. Is there anyone who has this kind of experience and know why? With best wishes, Richard
