Not much idea. I tend to build small amounts at the termini of your current model using the better model as a guide then re-refine and hope you can keep going.. Eleanor
On 2 October 2017 at 15:42, Vikram Dalal <[email protected]> wrote: > Hi all, > > > I am solving a protein data of 2.6 A. It has two monomers in ASU. It has > 377 residues. I have built residues 26 to 36 and 41 to 377 in A chain while > B chain has model 42 to 129, 146 to 168 and 189 to 371. Current R factor > and R Free is 23.2 and 28.5, respectively. I have attached electron > density (fourier map at 1.0 contour and difference map at 2.5) figures for > 130 to 145, 169 to 188 and C terminal region of B chain and superposed > model of A chain is shown in green color. > > I have tried manually building missing residues of B chain according to A > chain model, but R free increased to 29. > > Any suggestions will be highly aprreciated. > > > Thanks & Regards, > > > > >
