Dear all
Iodine gave a good anomalous signal at copper k-alpha
wavelength. Thank you everyone for your good suggestions. I could build the
model and refine it and bring the R-factor/ R-free 0.214/ 0.247 at the
resolution of 1.6A (with the previous data set without anomalous signal,
resolution 1.6A). Is there any way to reduce the R-factor/ R-free further.
Thanks
Regards
Yuvaraj
On Tue, Nov 14, 2017 at 3:28 PM, YUVARAJ I <[email protected]> wrote:
> Dear all
> What are the chemicals which give an anomalous signal at home
> source (Cu *K*α radiation) apart from Lathanides and Zinc acetate. What
> are the things I should taken care of to get sufficient anomalous signal to
> solve the structure.
>
> I have crystal data without anomalous signal at 1.76A resolution. What
> are theoretical ways possible to solve the structure when there is no
> homologous structures available in the PDB.
>
> Note: There is one cysteine and one methionine in 176 AA length protein.
>
> Thanks
> Yuvaraj
>
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