That depends on the quality of your data and your model! I play with refinement and rebuilding - check indicators v resolution., etc etc (I look at the <Fobs> v <Fcalc> plot from REFMAC - can show up problems with low resolution data.
Eleanor On 29 November 2017 at 12:46, YUVARAJ I <yuvee...@gmail.com> wrote: > Dear all > Iodine gave a good anomalous signal at copper k-alpha > wavelength. Thank you everyone for your good suggestions. I could build > the model and refine it and bring the R-factor/ R-free 0.214/ 0.247 at the > resolution of 1.6A (with the previous data set without anomalous signal, > resolution 1.6A). Is there any way to reduce the R-factor/ R-free further. > > Thanks > > Regards > Yuvaraj > > > > > > On Tue, Nov 14, 2017 at 3:28 PM, YUVARAJ I <yuvee...@gmail.com> wrote: > >> Dear all >> What are the chemicals which give an anomalous signal at home >> source (Cu *K*α radiation) apart from Lathanides and Zinc acetate. What >> are the things I should taken care of to get sufficient anomalous signal to >> solve the structure. >> >> I have crystal data without anomalous signal at 1.76A resolution. What >> are theoretical ways possible to solve the structure when there is no >> homologous structures available in the PDB. >> >> Note: There is one cysteine and one methionine in 176 AA length >> protein. >> >> Thanks >> Yuvaraj >> >> -- >> >> > > > -- > > >
