I agree with Roger Rowlett, try a room temp capillary mount.  Also ,what is
the magnification of the microscope/TV system you are using to center these
tiny crystals? Centering the loop is not enough.

James Phillips

On Fri, Dec 1, 2017 at 8:48 AM, Thomas, Leonard M. <[email protected]> wrote:

> Looking a bit closer are you sure the crystal is protein?  I see a couple
> of spots which could indicate a small molecule of some variety.
>
> Len
>
>
> On Nov 30, 2017, at 3:04 PM, Geny <[email protected]> wrote:
>
> ​
>
> Dear EveryOne,
> ​
> ​ I recently tried to collect the diffraction data, but unluckily, the
> diffraction patter is attached in this email. There is no any diffraction
> spots ​at all. It is my honor to ask experts worldwide why the crystal does
> this, and how to improve the quality of this kind of crystal. if you have
> any ideas, would you like to share?  Thank you so much and really
> appreciate it.
>
> Bests,
> Geny
>
>
> <diffraction pattern.png>
> <diffraction pattern.png>
>
>
> Leonard Thomas
> [email protected]
>
>
>
>

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