I agree with Roger Rowlett, try a room temp capillary mount. Also ,what is the magnification of the microscope/TV system you are using to center these tiny crystals? Centering the loop is not enough.
James Phillips On Fri, Dec 1, 2017 at 8:48 AM, Thomas, Leonard M. <[email protected]> wrote: > Looking a bit closer are you sure the crystal is protein? I see a couple > of spots which could indicate a small molecule of some variety. > > Len > > > On Nov 30, 2017, at 3:04 PM, Geny <[email protected]> wrote: > > β > > Dear EveryOne, > β > β I recently tried to collect the diffraction data, but unluckily, the > diffraction patter is attached in this email. There is no any diffraction > spots βat all. It is my honor to ask experts worldwide why the crystal does > this, and how to improve the quality of this kind of crystal. if you have > any ideas, would you like to share? Thank you so much and really > appreciate it. > > Bests, > Geny > > > <diffraction pattern.png> > <diffraction pattern.png> > > > Leonard Thomas > [email protected] > > > >
