Hi Yu, Microseeding along with additive screening could give a better result, because I encountered a situation once like this. Try higher concentration of protein with bigger drop volume during crystallisation.
Good luck Prem On Wed, Feb 14, 2018 at 4:16 PM, Huyton, Trevor <trevor.huy...@mpibpc.mpg.de > wrote: > Dear Yu, > > > > If the crystals are grown from salt then put the drop over water for a few > hours and dissolve them, then put it back over the reservoir solution and > recrystallize. > > Sometimes you can get better crystals this way. > > You could also try melting them with temperature and recrystallizing > > > > Best > > > > Trevor > > > > *From: *CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Yu Qiu < > yu....@sanofi.com> > *Reply-To: *"yu....@sanofi.com" <yu....@sanofi.com> > *Date: *Tuesday 13 February 2018 at 16:11 > *To: *"CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> > *Subject: *[ccp4bb] protein quasicrystals? > > > > Hi, > > > > I have been trying to crystallize a protein complex and keep getting > sphere shape crystals. The diffraction is around 3 angstrom, but looks like > multiple lattices. I am wondering if it could be a quasi crystal? Is there > anyone has such experience? > > > > Thanks, > > Yu > -- With kind regards, Prem Prakash PhD Research Scholar Protein Crystallography Lab Biosciences and Bioengineering IIT Bombay