Dear BB, I apologize for the off topic. But I strongly believe this is the right place to ask my question.
We are working on a protein that is 300 amino acids in length. After many efforts, we could solve the structure at 1.60 Å resolution. It took almost 10 months to get this crystal and we could not reproduce it. The maps are interpretable and we could model only till residue 190 and we could not see any density for the rest of the molecule. My question is- Does anyone has encountered such a structure with lots of missing density? I would appreciate your efforts if someone can send me few references describing such type of structures. Is there any chance that microbes can cleave the protein in the crystal drops? Thanks, Rajesh..
