The precision or significance of rfree depends on the number of reflections used to calculate it. No fixed rule for percentage exists. Tickle & Brunger have elaborated on this. References pp 625 in bmc. Depending on how many data overall you have and can set aside, for an overall rfree uncertainty of approx 1% several 100 to 1000 suffice. If you just use rfree as a qualitative tool, criteria are less stringent than using it for quantitative estimates.
Best br On Tue, Jul 10, 2018, 10:06 张士军 <[email protected]> wrote: > Dear all > > I am confusing the choose of free > R-flags recently. Rfactor means calculatted from reflection not used in > refinement,so what's big the > difference between different percentage of R-flags,like it's about 5% in ccp4 > -refmac, while it is > 10% in phenix-refinement,what's the difference between them and how they > affect the > Rwork and Rfree values when do refinement. Thanks a lot ! > > best regards > > shijun > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
