Yes, essential to test at room temperature without changing their buffer medium before getting worried! If they don’t diffract at RT, they are very very unlikely to diffract at cryotemperatures, whatever you do to them before hand! Best wishes Elspeth
From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Hughes, Jon Sent: 14 August 2018 11:11 To: [email protected] Subject: [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :( maybe it's the cryobuffer that's the problem (you didn't mention it). you could try to fish the crystals with minimal liquid attached by mounting them in oil rather than a cryobuffer. or you could test the native diffraction "in situ" (at room temperature in the drop): quite a few beamlines offer this possibility these days. best jon Von: CCP4 bulletin board [mailto:[email protected]] Im Auftrag von Careina Edgooms Gesendet: Dienstag, 14. August 2018 11:59 An: [email protected]<mailto:[email protected]> Betreff: [ccp4bb] crystals that dont diffract :( :( I got the most beautiful crystals I have ever seen and they don't diffract at all. Not poor diffraction, NO diffraction. Anyone know why this could be and how I can go about fixing it? I had three beautiful crystals and not one diffracted. I did leave them in the drop for about 3 weeks before harvesting and in liquid nitrogen for about a month before diffracting. Could that be a factor? If I regrew more beautiful crystals and diffracted straight away could that help? Careina ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
