Hi One other thing to try that someone with grey hair in your lab might know about - mount a "crystal" in a capillary and see if it diffracts at room temp. As long as you have a source and detector in your home lab, there's no need to go to a synchrotron and use their in situ facilities.
There are those of us who would contend that if your sample doesn't diffract, then it isn't a crystal, no matter how nice it looks (though it might have been one once...)! Harry -- Dr Harry Powell Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) On 14 Aug 2018, at 11:14, Elspeth Garman wrote: > Yes, essential to test at room temperature without changing their buffer > medium before getting worried! > If they don’t diffract at RT, they are very very unlikely to diffract at > cryotemperatures, whatever you do to them before hand! > Best wishes > Elspeth > > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Hughes, > Jon > Sent: 14 August 2018 11:11 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] AW: [ccp4bb] crystals that dont diffract :( :( > > maybe it's the cryobuffer that's the problem (you didn't mention it). you > could try to fish the crystals with minimal liquid attached by mounting them > in oil rather than a cryobuffer. or you could test the native diffraction "in > situ" (at room temperature in the drop): quite a few beamlines offer this > possibility these days. > best > jon > > Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von > Careina Edgooms > Gesendet: Dienstag, 14. August 2018 11:59 > An: CCP4BB@JISCMAIL.AC.UK > Betreff: [ccp4bb] crystals that dont diffract :( :( > > I got the most beautiful crystals I have ever seen and they don't diffract at > all. Not poor diffraction, NO diffraction. Anyone know why this could be and > how I can go about fixing it? I had three beautiful crystals and not one > diffracted. I did leave them in the drop for about 3 weeks before harvesting > and in liquid nitrogen for about a month before diffracting. Could that be a > factor? If I regrew more beautiful crystals and diffracted straight away > could that help? > Careina > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
