I just give an example of oil. Indeed, paraffin is also a very good option. In some cases (<=5% alcohol). Here is the difference I observed between paraffin and paratone oil. 1. Paraffin oil has low viscosity. Paratone oil is too sticky with high viscosity. Sometimes, I mix them with different ratio. 2. In the crystallization with high concentration of alcohol (>10%), I prefer paratone oil, which can form a bulky cover immediately. Paraffin oil may spread out and could not cover the drop completely.
The basic idea is to proved an effective shell to prevent solvent evaporation. On Tue, Aug 14, 2018 at 12:56 PM Patrick Loll <[email protected]> wrote: > I second (third?) what Tommi and Kevin said about using an oil to cover > the drop to slow evaporation (I like paraffin for this—not too viscous). > Here’s an additional nuance: Saturate the oil with the alcohol first, > before using it to cover the drop. > > > On 14 Aug 2018, at 2:58 PM, Thomas Krey <[email protected]> > wrote: > > > > Dear crystallization experts, > > > > We have 3D protein crystals grown from a microseed matrix screening > vapor diffusion experiment in either > > > > 15% (v/v) Reagent alcohol > > HEPES Na pH 7.5 > > 0.2 M MgCl2 > > > > or in > > > > 27% Isopropanol > > 0.18 M MgCl2 > > 90 mM HEPES Na pH 7.5 > > 10% Glycerol > > > > Upon opening the corresponding wells these crystals move quite a bit – > presumably due to the volatility of the alcohols. Does anyone have a good > suggestion to stabilize the swirling movements? Does anyone have > experience, whether these conditions alone can serve as cryo-protectant > (i.e., do we really have to fish, move into cryo solution and fish again)? > > Any suggestion or input would be highly welcome. > > > > Thank you very much in advance. > > > > Thomas > > > > > > Prof. Dr. Thomas Krey > > Hannover Medical School > > Institute of Virology > > Structural Virology Group > > Carl-Neuberg-Str. 1 > > D-30625 Hannover > > phone: +49 (0) 511 - 532 4308 > > email: [email protected] > > > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > > --------------------------------------------------------------------------------------- > Patrick J. Loll, Ph. D. > Professor of Biochemistry & Molecular Biology > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St., Mailstop 497 > Philadelphia, PA 19102-1192 USA > > (215) 762-7706 > [email protected] > [email protected] > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Kevin Jin Sharing knowledge each other is always very joyful...... Website: http://www.jinkai.org/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
