Dear Xiaodi 

 

The wwPDB validation service does indeed add hydrogens using Reduce before 
calculating clashes. 

For more details of what processes are run when generating the wwPDB validation 
report see the on-line help

https://www.wwpdb.org/validation/2017/XrayValidationReportHelp#close_contacts

 

If you would like us to look into this in more detail please can you email us 
directly on 

[email protected] <mailto:[email protected]> 

with details of your validation session.

 

This email address is also listed on the wwPDB validation help page

https://www.wwpdb.org/validation/validation-reports

 

Regards

 

John

 

From: CCP4 bulletin board <[email protected]> On Behalf Of Sharan Karade
Sent: 16 January 2019 04:28
To: [email protected]
Subject: Re: [ccp4bb] clashscore question

 

Dear Yu,

 

I think pdb validation server add hydrogen to the structure and then calculate 
the clashscore. The other methods you mentioned calculate clashscore without 
adding hydrogen to the structure.

 

Regards

Sharan

 

On Tue, Jan 15, 2019, 11:23 PM Xiaodi Yu <[email protected] 
<mailto:[email protected]>  wrote:

Hi All:

 

I have a pdb file and the clashscores reported from both phenix and MolProbity 
were around 5, while the clashscore from the pdb validation server is 17. I 
wonder what may cause the difference? Any suggestion is appreciated.

 

Thank you.

 

Xiaodi 

 

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