Dear CCP4-BB,
after refining a fairly high resolution aldose reductase structure
(0.96 A) we observed negative Fo-Fc density (-3.0 sigma) for the three
C-terminal residues (314-316). However, the 2Fo-Fc density clearly
showed the position of these residues without indication of a second
conformation for these residues. Therefore, we decided to refine the
occupancy for the entire 3 residues, which refined to 70%. After
occupancy refinement the negative density disappeared. We are now
courious if it is acceptable to deposit the structure with 3 residues
only partially occupied without indication of a second conformation or
how to proceed otherwise?
Thanks for any advice,
Lea and Andreas
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