Hi,
If the interaction between partners is of a hydrophobic nature and
involves leucines, it may be MPD destroys the interaction.
Have a look at the following publication:
Acta Crystallogr D Biol Crystallogr.
<https://www.ncbi.nlm.nih.gov/pubmed/12351894#>2002 Oct;58(Pt 10 Pt
1):1722-8. Epub 2002 Sep 26.
An overview on 2-methyl-2,4-pentanediol in crystallization and in
crystals of biological macromolecules.
Anand K
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Anand%20K%5BAuthor%5D&cauthor=true&cauthor_uid=12351894>^1
,Pal D
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Pal%20D%5BAuthor%5D&cauthor=true&cauthor_uid=12351894>,Hilgenfeld
R
<https://www.ncbi.nlm.nih.gov/pubmed/?term=Hilgenfeld%20R%5BAuthor%5D&cauthor=true&cauthor_uid=12351894>.
You will have to try and find a substitue for it.....
Good luck,
Jeroen
Am 01.02.19 um 17:42 schrieb Jorg Stetefeld:
2-Methyl-2,4-pentanediol (MPD)
--
*Dr.math. et dis. nat.Jeroen R. Mesters*
Deputy, Lecturer, Program Coordinator /Infection Biology
/
<http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html>Visiting
Professorship (South Bohemian University) in Biophysics
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*University of Lübeck*
Center for Structural and Cell Biology in Medicine
*Institute of Biochemistry*
Tel +49 451 3101 3105 (secretariate 3101)
Fax +49 451 3101 3104
jeroen.mest...@uni-luebeck.de <mailto:jeroen.mest...@uni-luebeck.de>
www.biochem.uni-luebeck.de <http://www.biochem.uni-luebeck.de>
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