As I understand helical assembly, the data some time is anisotropic (any one or two a*,b* or c* has more diffraction than other), which may be responsible for your high Rfactors. You can crop the data to the resolution using softwares.
Regards Sharan On Mon, Mar 25, 2019, 8:17 AM Andreas Förster <docandr...@gmail.com> wrote: > Yeah, good stuff: > > http://www.auspex.de/ > > All best. > > > Andreas > > > > On Mon, Mar 25, 2019 at 10:45 AM CCP4BB < > 0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote: > >> Even if you can't see them, it may be worthwhile investigating with >> Auspex (Parkhurst, Thorn, Winter & Waterman - sorry, I can't remember the >> reference off-hand). There's an easy to use webserver somewhere that runs >> it... >> >> Harry >> -- >> Dr Harry Powell >> >> On 25 Mar 2019, at 09:03, herman.schreu...@sanofi.com wrote: >> >> Dear ???, >> >> Do you have any ice rings (even hardly visible ones) in your diffraction >> data? >> >> Best, >> >> Herman >> >> >> >> *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK >> <CCP4BB@JISCMAIL.AC.UK>] *Im Auftrag von *StrBio >> *Gesendet:* Sonntag, 24. März 2019 05:17 >> *An:* CCP4BB@JISCMAIL.AC.UK >> *Betreff:* [EXTERNAL] [ccp4bb] Refinement >> >> >> >> ALL. >> >> >> >> I have data at 2.4 A in P21 sp gr, helical protein. >> >> Refined to Rwork 29 Rfree 34 with nice density map and all nice >> statistics oither Rfactor (by Phenix). Refmac quit same. >> >> Should I deposit it or look better data? >> >> Any suggestion? >> >> >> >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=oiqj1rjVNhpCcllUhUS_5AMqy40wZK8ZblEZ30G2kII&s=urg-c9RVUGGkc6JFD85DMrGGYINbAZShekVsZVrEvdA&e=> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
