I would try chloride and potassium in those sites and see whether that refines well.
Cheers, Robbie On 17 Apr 2019 07:49, WENHE ZHONG <wenhezhong.xmu....@gmail.com> wrote: Dear all, We found a huge density blob close to residues Arg and His (see attached) and another big density blob near three Thr residues (see attached) in the structure with an resolution of 2.6A. They are too big to place water atoms. In the crystallization condition, we have PEG 8000, glycerol, triethanolamine-HCl (TEA) buffer pH 7.2, KCl, MgCl2 and ATP. Do you have any idea about what can be in the density? Covalent modification? Thanks. Kind regards, Wenhe [cid:48C74A30-E0A3-4EA8-BFE6-E0EB418751C2@local.local][cid:E8DDA617-5B85-4883-AF05-E8175CC33F51@local.local] [cid:C3ADA69F-7A08-41E0-9B71-362BE360F09E@local.local][cid:5CFE7EF9-6FCD-4C69-941B-4A72B3776108@local.local] ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
