Lumbini, It would useful to know the distance between the sulfur and center of the Fo-Fc map that you have shown. Since you have very high resolution data, the extra density could be for a hydrogen atom in CSO-H (sufeneic acid) or anisotropy of the oxygen atom.
Anthony From: [email protected] To: [email protected] Sent: Tuesday, July 9, 2019 4:48:32 PM Subject: Re: [ccp4bb] Fo-Fc density close to cysteine residue Any anomalous diffraction? On Tue, 9 Jul 2019 at 10:32, Lumbini Yadav < [ mailto:[email protected] | [email protected] ] > wrote: Dear all, We have found a huge Fo-Fc density close to cysteine residue (see attached image) in the structure with resolution of 1.2A. In the crystallization condition, we have PEG 3350, Potassium phosphate monobasic, glycerol and protein was in Tris and NaCl. Before freezing the crystals were soaked in mother liquor containing sodium dithionite. I have tried different modified cysteine (CSX, CSO, OCS, CME, CSS, SNC, CSD, CXM, SCH, CSU) from the library and also SO 3 , SO 2 and peroxide. But in all the screenings we do see some part of Fo-Fc density unaddressed at 3 sigma. Does anyone have an idea about what this density could be? Covalent modification? Thanks. Kind regards, Lumbini To unsubscribe from the CCP4BB list, click the following link: [ https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 | https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ] To unsubscribe from the CCP4BB list, click the following link: [ https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 | https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
