Thanks for the reply. The distance between sulphur and centre of Fo-Fc map is around 2.5A. The density does not appear to be of hydrogen (attached image) anisotropy refinement also does not help.
On Tue, Jul 9, 2019 at 5:13 PM Anthony Addlagatta <[email protected]> wrote: > Lumbini, > > It would useful to know the distance between the sulfur and center of the > Fo-Fc map that you have shown. Since you have very high resolution data, > the extra density could be for a hydrogen atom in CSO-H (sufeneic acid) or > anisotropy of the oxygen atom. > > Anthony > ------------------------------ > *From: *[email protected] > *To: *[email protected] > *Sent: *Tuesday, July 9, 2019 4:48:32 PM > *Subject: *Re: [ccp4bb] Fo-Fc density close to cysteine residue > > Any anomalous diffraction? > > On Tue, 9 Jul 2019 at 10:32, Lumbini Yadav <[email protected]> wrote: > >> Dear all, >> >> >> >> We have found a huge Fo-Fc density close to cysteine residue (see >> attached image) in the structure with resolution of 1.2A. In the >> crystallization condition, we have PEG 3350, Potassium phosphate >> monobasic, glycerol and protein was in Tris and NaCl. Before freezing >> the crystals were soaked in mother liquor containing sodium dithionite. >> >> >> >> I have tried different modified cysteine (CSX, CSO, OCS, CME, CSS, SNC, CSD, >> CXM, SCH, CSU) from the library and also SO3, SO2 and peroxide. But in >> all the screenings we do see some part of Fo-Fc density unaddressed at 3 >> sigma. >> >> >> >> Does anyone have an idea about what this density could be? Covalent >> modification? >> >> >> >> Thanks. >> >> >> >> Kind regards, >> >> Lumbini >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
