Thanks everyone for your suggestions, I have a lot to try! Luke
On Tue, 23 Jul 2019 at 17:43, Eleanor Dodson <[email protected]> wrote: > Well - there are lots of ways to proceed. It doesnt really matter for the > crystallography theory if the exptl phases are from SAD or SIR. Just harder > to handle in some software > I think I would start to refine the poor MR model with the xptl phases as > restraints. > REFMAC will do this. Then see if the maps look better - can you see > features not in the model?.. > If they do you could use Buccaneer to try to rebuild the existing model > using exptl phases.. > It might work! > Eleanor > > On Tue, 23 Jul 2019 at 09:55, Luke Smithers < > [email protected]> wrote: > >> Hi all, >> >> I have collected native data and data on a bromide derivative of one of >> my crystals, but have struggled to get a second derivative. I went through >> the SIR pipeline as the anomalous signal from the Br was weak and have >> generated some not-so-great phases. I have also attempted MR with the >> closest match in the PDB (which is only 22% sequence identity) and got some >> more not-so-great phases. I have found plenty of programs that will combine >> SAD/MR phases, is there anything that will do SIR/MR? I understand it's >> likely I will need more and better data and ideally a second derivative but >> thought it was worth putting it out there to see if there is something like >> this. >> >> Luke >> >> -- >> >> *Luke Smithers* >> >> *PhD Candidate* >> >> Protein Production and Structure Facility, School of Molecular Sciences >> >> *T *+61 8 6488 3163 >> >> [image: The University of Western Australia] >> <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/uwa-logo/> >> >> [image: Pursue Impossible] >> <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/pursue>[image: >> Facebook] >> <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/facebook>[image: >> Twitter] >> <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/twitter>[image: >> Youtube] >> <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/youtube> >> >> [image: >> http://static.weboffice.uwa.edu.au/visualid/graphics/email/sig2015/campaign.gif] >> <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/campaign> >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > -- *Luke Smithers* *PhD Candidate* Protein Production and Structure Facility, School of Molecular Sciences *T *+61 8 6488 3163 [image: The University of Western Australia] <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/uwa-logo/> [image: Pursue Impossible] <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/pursue>[image: Facebook] <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/facebook>[image: Twitter] <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/twitter>[image: Youtube] <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/youtube> [image: http://static.weboffice.uwa.edu.au/visualid/graphics/email/sig2015/campaign.gif] <http://www.uwa.edu.au/university-campaigns-resources/emailsig2015/campaign> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
