Unlimited length in an hour is physically unlikely, but the rest is not that far off I think.
We are at or below 1 cent per basepair already (in bulk)... Artem On Wed, Jul 24, 2019, 13:01 Keller, Jacob <[email protected]> wrote: > Yes, imagine 1 hr, tabletop, unlimited-length, error-free, dirt-cheap gene > synthesis! (With a parallized option for 96-well plates, of course...) > > We'll probably just have to wait 10 years. > > JPK > > +++++++++++++++++++++++++++++++++++++++++++++++++ > Jacob Pearson Keller > Research Scientist / Looger Lab > HHMI Janelia Research Campus > 19700 Helix Dr, Ashburn, VA 20147 > Desk: (571)209-4000 x3159 > Cell: (301)592-7004 > +++++++++++++++++++++++++++++++++++++++++++++++++ > > The content of this email is confidential and intended for the recipient > specified in message only. It is strictly forbidden to share any part of > this message with any third party, without a written consent of the sender. > If you received this message by mistake, please reply to this message and > follow with its deletion, so that we can ensure such a mistake does not > occur in the future. > > -----Original Message----- > From: CCP4 bulletin board <[email protected]> On Behalf Of > Hargreaves, David > Sent: Wednesday, July 24, 2019 6:01 AM > To: [email protected] > Subject: Re: [ccp4bb] challenges in structural biology > > Dear CCP4bb, > > From my industrial perspective: The crystallisation bottleneck has > probably been fairly well addressed. High throughput screening using > robotics and around 20ul of protein per screen is common. There are lots of > screens to try even if they are rather redundant in their content. However, > I feel there are two things missing: high throughput protein production at > microscale and specific tools for construct design. Generally, industry is > interested in the druggable site which perhaps is only one domain of a > multidomain target protein. Getting a suitably robust crystal system often > requires more than one iteration of multiple construct design which in my > experience is something of a lottery. Some helpful design tools and an > order of magnitude increase in the number of constructs that can be > delivered from protein supply would help. > > Best wishes, > > David > > > Dr. David Hargreaves > Associate Principal Scientist > _____________________________________________________________________ > AstraZeneca > Discovery Sciences, Structure & Biophysics Dr David Hargreaves. > Office R1-09/Lab FL54 > AstraZeneca Darwin Building > Unit 310 > Cambridge Science Park > Milton Road > Cambridge > CB4 0WG > > telephone: +441223223546 > > David.Hargreaves @astrazeneca.com > > Please consider the environment before printing this e-mail > > ________________________________ > > > AstraZeneca UK Limited is a company incorporated in England and Wales with > registered number:03674842 and its registered office at 1 Francis Crick > Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0AA. > > This e-mail and its attachments are intended for the above named recipient > only and may contain confidential and privileged information. If they have > come to you in error, you must not copy or show them to anyone; instead, > please reply to this e-mail, highlighting the error to the sender and then > immediately delete the message. For information about how AstraZeneca UK > Limited and its affiliates may process information, personal data and > monitor communications, please see our privacy notice at > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.astrazeneca.com&d=DwIGaQ&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=h6L9Puq8CXUJwM-9E9EErRjZQ1EoglqehbJ03ZP2g_U&s=95JD_SJicftV_XBNeJniuudTPWh9DIp7f6UdpPhO0F8&e= > < > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.astrazeneca.com&d=DwIGaQ&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=h6L9Puq8CXUJwM-9E9EErRjZQ1EoglqehbJ03ZP2g_U&s=lmVT9E-QgTO8z9MZH5gAiQJM6a70IwXFlC5KPhLXhfM&e= > > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwIGaQ&c=LU6cRtx0xgB8s29tIz9Olw&r=eLCg9eJ4Rs_LnxfUWsp7FSxhIEcZYmTSU4Uyq1bRYPI&m=h6L9Puq8CXUJwM-9E9EErRjZQ1EoglqehbJ03ZP2g_U&s=sNFvm1DPLLfFpAAch2LN6TrwU6EYoiEr6an6TmFblhI&e= > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
