I have some Seleno-Methionine protein crystals (12 SeMet of 211 amino acids, incorporation verified by Mass Spec). I've already collected several datasets (ALS BL5.0.2) but I seem to be losing (rejecting?) a lot of anomalous signal during data processing. I'm most familiar with HKL2000, but I have tried XDS and DIALS auto-processing. Here is a scan: https://ibb.co/LZqm33p and here is an example of a frame: https://ibb.co/gR3ZR47. Each frame is 0.25° and I'm using inverse beam with wedge size 1°. Maybe I need to adjust my collection strategy? All previous datasets have been in space group P 21 with dimensions of approx. 24.5Å, 85Å, 40Å, 90°, 96.5°, 90°. I'm sure there are additional things I can be doing in HKL but I've run out of ideas. Any advice or recommendations would be appreciated. Please let me know if you need additional information.
Thank you, Lindsey ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
