Hi Lindsey, I do agree with the others on improving your experimental strategy to enhance the anomalous signal in your data, but did you try to solve the structure with the data you already have? I have seen cases where there is only a pathetic anomalous signal from fully Se-Met incorporated crystals in any experimental setting and those could eventually be solved. You probably have to deal with a significant number of false positives in your substructure but if the anomalous signal is observed accurately you are quite likely able to solve the structure with weak data alone.
How much anomalous signal did you get out of those frames and how much did you expect to get? Have you tried merging data from several crystals collected at the same wavelength ? Best, Stefan ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
