Dear Natesh, as Petri pointed out this is a common behavior for proteins/protein complexes that deviate substantially from a "globular" shape. It is very typical for elongated proteins/protein complexes containing e.g. extended coiled-coil regions. These samples will elute much earlier from a SEC column than expected from their molecular weight, seemingly suggesting oligomerisation is some form. MALS will allow you to unambigously characterize their molecular weight independent of their hydrodynamic radius.
See e.g. https://elifesciences.org/articles/42129 for a recent example. You will find many if you search for coiled-coil structures or other motifs that impose elongated shapes. Best, Arjen On Tue, 27 Aug 2019 at 07:57, Natesh Ramanathan <[email protected]> wrote: > Dear Friends, > > Can you share your experience with examples of MALS giving lower > molecular weight (Eg. Monomer) and SEC giving higher molecular weight (Eg. > Dimer), for the same protein sample? > > If you have/know any published paper, can you please point me to > that reference paper or send me the paper? > > Many thanks. > Best regards, > Natesh > > > -- > ---------------------------------------------------------- > "Live Simply and do Serious Things .. " > - Dorothy Mary Crowfoot Hodgkin OM, FRS > > "In Science truth always wins" > - Max Ferdinand Perutz OM FRS > ---------------------------------------------------------- > Dr. Ramanathan Natesh > Assistant Professor, > School of Biology, > Indian Institute of Science Education and Research Thiruvananthapuram > (IISER-TVM), > Maruthamala P.O., Vithura, > Thiruvananthapuram, 695551, Kerala, India > > [email protected] > http://www.researcherid.com/rid/C-4488-2008 > ORCID: http://orcid.org/0000-0002-1145-5962 > https://publons.com/author/1520837/ramanathan-natesh#profile > http://faculty.iisertvm.ac.in/natesh > > Office Ph. 0091- 471-2778087 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
