Hi everyone –
I highly recommend Vladimir Uversky’s publications on the quantitative analysis of intrinsically disordered and flexible proteins by SEC. >From my experiences across many projects using SEC-MALS, what is described >here is a very common occurrence (and very commonly misunderstood). Kushol Kushol Gupta, Ph.D. Research Assistant Professor, <https://www.med.upenn.edu/biocbiop/index.html> Department of Biochemistry and Biophysics Director, <https://www.med.upenn.edu/jf/bsbcore/index.html> Johnson Foundation Structural Biology and Biophysics Core <http://www.med.upenn.edu/> Perelman School of Medicine at <http://www.upenn.edu/> The University of Pennsylvania <mailto:[email protected]> [email protected] / 267.259.0082 / <http://www.stwing.upenn.edu/~kgupta> www.stwing.upenn.edu/~kgupta From: CCP4 bulletin board <[email protected]> On Behalf Of arjen jakobi Sent: Tuesday, August 27, 2019 4:56 AM To: [email protected] Subject: Re: [ccp4bb] SEC and MALS Dear Natesh, as Petri pointed out this is a common behavior for proteins/protein complexes that deviate substantially from a "globular" shape. It is very typical for elongated proteins/protein complexes containing e.g. extended coiled-coil regions. These samples will elute much earlier from a SEC column than expected from their molecular weight, seemingly suggesting oligomerisation is some form. MALS will allow you to unambigously characterize their molecular weight independent of their hydrodynamic radius. See e.g. https://elifesciences.org/articles/42129 for a recent example. You will find many if you search for coiled-coil structures or other motifs that impose elongated shapes. Best, Arjen On Tue, 27 Aug 2019 at 07:57, Natesh Ramanathan <[email protected] <mailto:[email protected]> > wrote: Dear Friends, Can you share your experience with examples of MALS giving lower molecular weight (Eg. Monomer) and SEC giving higher molecular weight (Eg. Dimer), for the same protein sample? If you have/know any published paper, can you please point me to that reference paper or send me the paper? Many thanks. Best regards, Natesh -- ---------------------------------------------------------- "Live Simply and do Serious Things .. " - Dorothy Mary Crowfoot Hodgkin OM, FRS "In Science truth always wins" - Max Ferdinand Perutz OM FRS ---------------------------------------------------------- Dr. Ramanathan Natesh Assistant Professor, School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram (IISER-TVM), Maruthamala P.O., Vithura, Thiruvananthapuram, 695551, Kerala, India [email protected] <mailto:[email protected]> http://www.researcherid.com/rid/C-4488-2008 ORCID: <http://orcid.org/0000-0002-1145-5962> http://orcid.org/0000-0002-1145-5962 https://publons.com/author/1520837/ramanathan-natesh#profile http://faculty.iisertvm.ac.in/natesh Office Ph. 0091- 471-2778087 _____ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> &A=1 _____ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> &A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
