Hello, We have a bit tricky case of coiled coil protein with good data (2.05Å) for dimeric coiled coil (dimer in AU) - looks like AMPLE might be a way to solve such cases, if you know other good programs please suggest (Better yet if there is a clear how-to manual)
Some technical tips on usage for generation of fragments for AMPLE would be of help, not completely on top of that… (running the QUARK server, the real sequence is bit over 200 aa so not sure what is the best approach here? Rosetta? any how-to for that.. well i am running Robetta fragments too). with AMPLE can I do this with the online server or better run locally (need Rosetta installed I take it?) Suppose I could try Rosetta-MR also, but to my recollection that requires some kind of a phaser hit first to be improved, and i dont think I am there. Thanks for any comments, Best, Tommi ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
