On 10/12/2019 15:11, Sudipta Bhattacharyya wrote: > Dear community, > > I would really appreciate your suggestions for linking cysteine > sulfenic acid (one of the active site amino acid residues in my target > protein) to rest of the protein chain. Let me summarize what I did so > far in coot - > > 1. I deleted the native cysteine residue at that location, then > brought cysteine sulfenic acid from the monomer library, then fit it > in the density and merged it, then ran refinement by refmac5, the > entity fits really well but the link is not established after the > refinement .
What is the group of the cysteine sulfenic acid? If you tell coot that it is not a polymer, it will believe you. > > 2. In my version of coot (wincoot) I could not find "Extensions > > Modelling > Replace Residue"...option to change it. > Information about WinCoot binaries is available here: http://bernhardcl.github.io/coot/wincoot-download.html Regards, Paul. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
