What’s your spacegroup ? RWork / RFree? Twinning by any chance?
Jürgen __________________________________________ Jürgen Bosch, Ph.D. Division of Pediatric Pulmonology and Allergy/Immunology Case Western Reserve University 2109 Adelbert Rd, BRB 835 Cleveland, OH 44106 Phone: 216.368.7565 Fax: 216.368.4223 https://www.linkedin.com/in/jubosch/ CEO & Co-Founder at InterRayBio, LLC Johns Hopkins University Bloomberg School of Public Health Department of Biochemistry & Molecular Biology > On Dec 16, 2019, at 1:50 PM, Jessica Besaw <[email protected]> wrote: > > I am crystallizing this membrane protein in a medium (bicelles) that forms > lamella like sheets that stack on top of each other. > > The layer packing is shown below. Is this structure unreasonable? > > <Screen Shot 2019-12-16 at 1.40.02 PM.png> > > On Mon, 16 Dec 2019 at 13:38, Reza Khayat <[email protected] > <mailto:[email protected]>> wrote: > Hi Jessica, > > > > The gap between the two proteins is a bit troubling. Perhaps it's the image, > but why would a crystal form if there is no crystal contact between the two > proteins? > > > > Reza > > > > Reza Khayat, PhD > Assistant Professor > City College of New York > Department of Chemistry > New York, NY 10031 > From: CCP4 bulletin board <[email protected] > <mailto:[email protected]>> on behalf of Ashish Kumar > <[email protected] <mailto:[email protected]>> > Sent: Monday, December 16, 2019 1:24 PM > To: [email protected] <mailto:[email protected]> > Subject: [EXTERNAL] Re: [ccp4bb] 1 out of 2 proteins in asymmetric unit does > not fit density > > Hi Jessica, > > It may be possible because of wrong MR solution as well. How were your stats > after MR. > Also it is correct that it could be possible because of wrong space group. > Try changing the Space group and repeat MR. > > Best Regards > Ashish > > On 16 Dec 2019 22:56, "Jessica Besaw" <[email protected] > <mailto:[email protected]>> wrote: > Dear community, > > I am having a lot of trouble solving a protein structure. I think my problem > may caused by incorrectly placed proteins in molecular replacement. I have > two proteins in my asymmetric unit. It appears that one protein fits > perfectly, and the other one has many errors. (See snapshots below). I have > tried deleting the parts of the protein (and even the whole protein) to try > and rebuild it in COOT, but it was a bit too difficult for me to solve. > > I would appreciate any and all suggestions for potential strategies moving > forward. > > Other information: > (1) 2.4 Angstrom > (2) 99% complete > (3) "Translational NCS may be present at a level that may complicate > refinement" > > Cheers! > > Jessica > > <Screen Shot 2019-12-16 at 11.58.10 AM.png> > <Screen Shot 2019-12-16 at 11.58.31 AM.png> > > > > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=MBjWbF0ZDC5tg1IQYg3-zjOPSn7yuF2KfXIxak9l0MA&s=E2hs1sz4cNOm0vRwjsoHzxqEyBnMO-5BfM18hOltqLI&e=> > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=MBjWbF0ZDC5tg1IQYg3-zjOPSn7yuF2KfXIxak9l0MA&s=E2hs1sz4cNOm0vRwjsoHzxqEyBnMO-5BfM18hOltqLI&e=> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
