There have been two potential space groups: P212121 - Rfree = 36% P21212 - Rfree = 45%
Xtriage reports that twinning is unlikely. Cheers! Jessica On Mon, 16 Dec 2019 at 13:56, Jürgen Bosch <[email protected]> wrote: > What’s your spacegroup ? RWork / RFree? > > Twinning by any chance? > > Jürgen > > __________________________________________ > Jürgen Bosch, Ph.D. > Division of Pediatric Pulmonology and Allergy/Immunology > Case Western Reserve University > 2109 Adelbert Rd, BRB 835 > Cleveland, OH 44106 > Phone: 216.368.7565 > Fax: 216.368.4223 > https://www.linkedin.com/in/jubosch/ > > CEO & Co-Founder at InterRayBio, LLC > > Johns Hopkins University > Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > > On Dec 16, 2019, at 1:50 PM, Jessica Besaw <[email protected]> wrote: > > I am crystallizing this membrane protein in a medium (bicelles) that forms > lamella like sheets that stack on top of each other. > > The layer packing is shown below. Is this structure unreasonable? > > <Screen Shot 2019-12-16 at 1.40.02 PM.png> > > On Mon, 16 Dec 2019 at 13:38, Reza Khayat <[email protected]> wrote: > >> Hi Jessica, >> >> >> The gap between the two proteins is a bit troubling. Perhaps it's the >> image, but why would a crystal form if there is no crystal contact between >> the two proteins? >> >> >> Reza >> >> >> Reza Khayat, PhD >> Assistant Professor >> City College of New York >> Department of Chemistry >> New York, NY 10031 >> ------------------------------ >> *From:* CCP4 bulletin board <[email protected]> on behalf of Ashish >> Kumar <[email protected]> >> *Sent:* Monday, December 16, 2019 1:24 PM >> *To:* [email protected] >> *Subject:* [EXTERNAL] Re: [ccp4bb] 1 out of 2 proteins in asymmetric >> unit does not fit density >> >> Hi Jessica, >> >> It may be possible because of wrong MR solution as well. How were your >> stats after MR. >> Also it is correct that it could be possible because of wrong space group. >> Try changing the Space group and repeat MR. >> >> Best Regards >> Ashish >> >> On 16 Dec 2019 22:56, "Jessica Besaw" <[email protected]> wrote: >> >> Dear community, >> >> I am having a lot of trouble solving a protein structure. I think my >> problem may caused by incorrectly placed proteins in molecular replacement. >> I have two proteins in my asymmetric unit. It appears that one protein fits >> perfectly, and the other one has many errors. (See snapshots below). I have >> tried deleting the parts of the protein (and even the whole protein) to try >> and rebuild it in COOT, but it was a bit too difficult for me to solve. >> >> I would appreciate any and all suggestions for potential strategies >> moving forward. >> >> Other information: >> (1) 2.4 Angstrom >> (2) 99% complete >> (3) "Translational NCS may be present at a level that may complicate >> refinement" >> >> Cheers! >> >> Jessica >> >> <Screen Shot 2019-12-16 at 11.58.10 AM.png> >> <Screen Shot 2019-12-16 at 11.58.31 AM.png> >> >> >> >> >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=MBjWbF0ZDC5tg1IQYg3-zjOPSn7yuF2KfXIxak9l0MA&s=E2hs1sz4cNOm0vRwjsoHzxqEyBnMO-5BfM18hOltqLI&e=> >> >> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=MBjWbF0ZDC5tg1IQYg3-zjOPSn7yuF2KfXIxak9l0MA&s=E2hs1sz4cNOm0vRwjsoHzxqEyBnMO-5BfM18hOltqLI&e=> >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
