Hello,
clearly off-topic but maybe someone here is experienced in the analysis of array scans and can offer some advice? That would be beautiful. Here’s the problem: I want to test an ELISA-based HT array for phosphorylation profiling of a whole pathway, with almost 200 antibodies at once. The array company offers free scanning of the arrays, i.e. we do the experiment, sent the slides back, and receive the scans as raw images in tiff format. Can anyone suggest a suitable program to analyse such images of array scans on a Mac? There are six replicates per antibody per slide so in theory a good basis for at least semi-quantitative analysis - but finding a program to analyse these data is a real pain. The scans are obtained with a GenePix scanner I am being told but the instrument’s software does not run on a Mac. I tried ImageJ, which is open source and runs but needs some plugin for arrays that does not work for me (I suspect it worked for a previous version of ImageJ). I also tried TIGR Spotfinder, which in theory seems the perfect program (plenty of documentation), is also open source and Mac compatible - but when I try to compile it several files seem missing from the sourceforge package (and I cannot find another source). Does anyone use either ImageJ with the array plugin or the Spotfinder and can assure me that these options are still being developed or maybe knows for sure they are dead and not worth investing any more time? Or, even better, could point me to a program that runs on a Mac and is suitable for array analysis (it does not actually have to be free as long as it works for users who do not write code). I do have a GAL file for the images. Many thanks for your help and sorry for the spam! Bärbel -- Bärbel Blaum, PhD Inthera Bioscience AG Einsiedlerstrasse 34 CH-8820 Waedenswil Switzerland E-Mail: [email protected] Phone: +41 43 477 94 72-- ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
