Hello,
You could run Image Quant TL in a VM (parallels, vmware or virtual box).
https://bmi.cchmc.org/resources/software/imagequant-tl
For this appliation (arrays), we use an old program called VisualGrid
that is no longer available and run it an isolated XP VM via virtual box
(in linux).
Darren
On 21/01/2020 15:48, Bärbel Blaum wrote:
Hello,
clearly off-topic but maybe someone here is experienced in the
analysis of array scans and can offer some advice? That would be
beautiful. Here’s the problem: I want to test an ELISA-based HT array
for phosphorylation profiling of a whole pathway, with almost 200
antibodies at once. The array company offers free scanning of the
arrays, i.e. we do the experiment, sent the slides back, and receive
the scans as raw images in tiff format. Can anyone suggest a suitable
program to analyse such images of array scans on a Mac? There are six
replicates per antibody per slide so in theory a good basis for at
least semi-quantitative analysis - but finding a program to analyse
these data is a real pain. The scans are obtained with a GenePix
scanner I am being told but the instrument’s software does not run on
a Mac. I tried ImageJ, which is open source and runs but needs some
plugin for arrays that does not work for me (I suspect it worked for a
previous version of ImageJ). I also tried TIGR Spotfinder, which in
theory seems the perfect program (plenty of documentation), is also
open source and Mac compatible - but when I try to compile it several
files seem missing from the sourceforge package (and I cannot find
another source).
Does anyone use either ImageJ with the array plugin or the Spotfinder
and can assure me that these options are still being developed or
maybe knows for sure they are dead and not worth investing any more
time? Or, even better, could point me to a program that runs on a Mac
and is suitable for array analysis (it does not actually have to be
free as long as it works for users who do not write code). I do have a
GAL file for the images.
Many thanks for your help and sorry for the spam!
Bärbel
--
Bärbel Blaum, PhD
Inthera Bioscience AG
Einsiedlerstrasse 34
CH-8820 Waedenswil
Switzerland
E-Mail: [email protected]
Phone: +41 43 477 94 72--
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--
**********************************************************************
Dr. Darren J. Hart,
CNRS Research Director, Institut de Biologie Structurale (IBS)
Unité Mixte de Recherche UMR5075 (CEA-CNRS-UGA)
Director, Integrated Structural Biology Grenoble (ISBG)
Unité Mixte de Service UMS3518 (CNRS-UGA-CEA-EMBL)
**********************************************************************
Email: [email protected]
Tel: +33 4 57 42 85 86
Physical address: IBS/ISBG, 71 avenue des Martyrs, 38000 Grenoble, France
Postal address: IBS/ISBG, 71 avenue des Martyrs, CS 20192, 38042
Grenoble, Cedex 9, France
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