Job description Dr. Julien’s laboratory at The Hospital for Sick Children Research Institute (Toronto, Canada) is looking for two Postdoctoral Fellows to join our Structural Biology team. By using a combination of biochemical, biophysical, immunological and structural techniques, the successful candidates will participate in the characterization of 1) immune receptors recognized by therapeutic antibodies, and 2) immune complexes of malaria antigens and human inhibitory antibodies. We are looking for driven and enthusiastic scientists who demonstrate passion and creativity towards the structure/function study of important disease targets and who can thrive in a highly-collaborative setting.
Roles & Responsibilities * Design and optimize constructs for expression in E.coli, insect cells (Sf9) and mammalian cells (HEK 293F); * Use Cryo-EM to determine the molecular structures of antigen-antibody complexes; * Determine X-ray crystal structures of antigens and antigen-antibody complexes; * Perform ITC and BLI binding assays between antigens and purified antibodies to determine binding thermodynamics and kinetics; * Perform protein engineering to develop antibody therapeutics and immunogens using structure-based approaches; * Biophysically characterize purified antigens for their Tm, Tagg and polydispersity. Desired Skills and Experience * Structural Biologist with a PhD in Biochemistry, Immunology, Molecular Biology, or related field; * Expertise in Cryo-EM and/or X-ray crystallography; * Experience in molecular biology, protein expression in various systems, purification and biophysical characterization (Octet, ITC, SEC-MALS, accelerated stability); * Strong knowledge in using structural biology to study pathogens and/or immune responses, especially in a context of structure-based antibody and vaccine design; * Practical experience in using in silico approaches for protein engineering is a plus; * Demonstrated ability to participate in collaborative scientific projects; * Organization skills, efficiency and meticulous record keeping. About Us Dr. Julien's laboratory is interested in the structure/function study of immune receptors and their interactions with antigens and therapeutics. A better understanding of the structures of B cell surface molecules will serve towards the rational design of improved biologics and vaccine immunogens against a variety of pathogens, including malaria and HIV-1. https://lab.research.sickkids.ca/julien/ We are located in The Peter Gilgan Centre for Research and Learning, a new 21-story, 750,000 square-foot facility that incorporates an open-concept design, state-of-the-art research facilities, and collaborative work spaces. As a member of the SickKids community, you will become part of a world-renowned organization dedicated to fulfilling a vision of Healthier Children, A Better World. For over 135 years, SickKids has worked hard to make the world a better place for children and their families. SickKids researchers have developed Pablum, discovered the gene for cystic fibrosis and are on the leading edge of research in a wide range of disciplines. Each day, people around the organization are doing meaningful work that directly, and often indirectly, benefits children in Canada and around the world. Please apply before Jan 31 by sending a CV and cover letter to: jean-philippe.jul...@sickkids.ca<mailto:jean-philippe.jul...@sickkids.ca>. Anticipated start date in Spring/Summer 2020. _______________________________________________________ Jean-Philippe Julien, Ph.D. Canada Research Chair in Structural Immunology Member, College of the Royal Society of Canada CIFAR Azrieli Global Scholar, Molecular Architecture of Life Senior Scientist, Program in Molecular Medicine, The Hospital for Sick Children Research Institute Assistant Professor, Departments of Biochemistry and Immunology, University of Toronto Office and Mailing address: The Hospital for Sick Children Research Institute Peter Gilgan Centre for Research and Learning (PGCRL), Room 20-9703 686 Bay St. Toronto ON Canada M5G 0A4 Office: 416-813-7654 ext309424 (Internal 309424) Lab: 416-813-7654 ext309425 (Internal 309425) Fax: 416-813-5993 (Internal 305993) jean-philippe.jul...@sickkids.ca<mailto:jean-philippe.jul...@sickkids.ca> https://lab.research.sickkids.ca/julien/ ________________________________ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
