Rosetta will do this. But it's not a server, and there is a fairly steep learning curve.
Artem - Cosmic Cats approve of this message On Mon, May 4, 2020 at 6:04 AM Firdous Tarique <kahkashantari...@gmail.com> wrote: > Hi > > I am trying to incorporate novel disulfide bond by introducing Cys > mutants at the interface of a protein complex (A+B) and (C+B) whose crystal > structure are already known. The idea is to introduce novel disulfide bonds > in these individual complexes which does not dissociate and can be tried > for cryo-EM studies for a larger complex (A+B+C) . As discussed > previously in the bb I am using 'Disulfide by Design" server to introduce > new Cys residues at the interface of the A+B and C+B The server suggested a > number of cys residues at the interface and now I am confused from where to > start with. Is there any known server which can predict the stability of > A+B and C+B with different disulfide bonds in it, making it easier to > decide and pick. In silico mutants which makes the complex more stable will > be chosen for site directed mutagenesis and downstream biochemical > experiments to check the stability of A+B+C. > > Thanks > > Kahkashan > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
