Hi I am trying to incorporate novel disulfide bond by introducing Cys mutants at the interface of a protein complex (A+B) and (C+B) whose crystal structure are already known. The idea is to introduce novel disulfide bonds in these individual complexes which does not dissociate and can be tried for cryo-EM studies for a larger complex (A+B+C) . As discussed previously in the bb I am using 'Disulfide by Design" server to introduce new Cys residues at the interface of the A+B and C+B The server suggested a number of cys residues at the interface and now I am confused from where to start with. Is there any known server which can predict the stability of A+B and C+B with different disulfide bonds in it, making it easier to decide and pick. In silico mutants which makes the complex more stable will be chosen for site directed mutagenesis and downstream biochemical experiments to check the stability of A+B+C.
Thanks Kahkashan ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
