hi alan,
why do you want to put the thing in a cold cabinet? if you have protease 
problems, there are better ways to get rid of them than exploiting Q10. on the 
other hand, you'll increase viscosity and thus slow down the flow rates. also, 
the high humidity will reduce the lifetime of your Äkta dramatically (ours has 
been running almost continuously for 20 years at room temperature and is still 
going strong [thanks to tina!]). if you really to need to keep the sample at 4 
°C, you can put the buffer(s) on ice and cool the column with a jacket.
best
jon

Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> Im Auftrag von Paula Salgado
Gesendet: Sonntag, 21. Juni 2020 18:50
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] Cold cabinet recommendations for AKTA Pure

Hi Alan

We got one a few years back, from Fisher, although I think Thermo are the 
manufacturer's:

Refrigerator FRCR4504W Forma chromatography THERMO SCIENTIFIC FORMA FRCR4504W

They also arranged for a heavy metal shelf to hold the AKTA as the normal 
shelves won't hold the weight.

We only have one AKTA Pure there, with a fraction collector and the rest of the 
cabinet holds all the columns.

Hope that helps
Paula


===================================

Dr Paula S. Salgado
Senior Lecturer in Macromolecular Crystallography
Molecular Mechanisms of Life Lead
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Newcastle University Biosciences Institute
Faculty of Medical Sciences
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Newcastle University
Newcastle upon Tyne, NE2 4HH, UK

Tel: +44 (0)191 208 7432
Email: paula.salg...@ncl.ac.uk<mailto:paula.salg...@ncl.ac.uk>


________________________________
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> 
on behalf of Alan Cheung <alan.che...@ucl.ac.uk<mailto:alan.che...@ucl.ac.uk>>
Sent: 21 June 2020 08:12
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> 
<CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: [ccp4bb] Cold cabinet recommendations for AKTA Pure


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Dear All

Apologies for being wildly off topic, but we're trying to find a decent cold 
cabinet for 2x AKTA Pure + F9C + sample pump.  Does anyone have any 
recommendations, especially for sliding door models?

Cheers,
Alan

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