Alexander Blake wrote a nice chapter on small-molecule crystallization in this book, if you run into problems in the crystallization stage:
Alexander Blake. Crystal growth and evaluation (Chapter 3). In Clegg, William (Ed.) Crystal Structure Analysis: Principles and Practice. 2nd Edition. IUCr/Oxford, 2009 <https://www.oxfordscholarship.com/view/10.1093/acprof:oso/9780199219469.001.0001/acprof-9780199219469>; <https://global.oup.com/academic/product/crystal-structure-analysis-9780199219476>. With the detector almost but not quite hitting the detector, one can typically collect all the data one needs on a home Cu source. Overloads can often be a problem---as has been pointed out out for synchrotrons---even on a home source. But they can usually be dealt with easily by modifying exposure, collecting lower- and higher-angle data separately, leaving some overlap between the two regions (see "Intensity level", Blake, Ch. 6.7.1, p. 88 in book above) and scaling. In extreme cases, one may need to lower the generator power. The nice thing about the slow pace of data collection on a home source is that one can think while doing the collection, thus catching and correcting most problems before it's too late. In Ch. 7.6 in the book, Blake also discusses some other problem cases. (The whole book's a great read.) Cheers, Navdeep >>> --- >>> On 01.06.20 23:50, Jiyuan Ke wrote: >>> > Hi Everyone, >>> > >>> > I want to crystallize a small organic molecule. I have >>> very limited >>> > experience in small molecule crystallography. I found >>> that the Crystal >>> > Screen HT from the Hampton research is good for both >>> small molecule and >>> > macromolecule crystallization. Plan to set up a sitting >>> drop screen just >>> > like setting up protein crystallization. I don’t know >>> if this is the >>> > proper way to do it. Is the MRC sitting drop 2-well >>> plate (HR3-083) used >>> > for protein crystallization good for small molecule >>> crystallization? Are >>> > there any special plates used for small molecule >>> crystallization? Is >>> > room temperature ok or not? >>> > >>> > For data collection, can I use the beamline for protein >>> crystals to >>> > collect data on small molecule crystals? Larger >>> oscillation angle, >>> > shorter exposure, reduced beam intensity? >>> > >>> > For structure determination, is SHELXL the preferred >>> software for >>> > solving small molecule structures? >>> > >>> > If anyone has experience in small molecule >>> crystallography, please >>> > help. Thanks! >>> > >>> > Best Regards, >>> > >>> > -- >>> > >>> > *Jiyuan Ke, Ph.D.* >>> > >>> > * >>> > * >>> > >>> > Research Investigator >>> > >>> > H3 Biomedicine Inc. >>> > >>> > 300 Technology Square, Floor 5 >>> > >>> > Cambridge, MA 02139 >>> > >>> > Phone: 617-252-3923 >>> > >>> > Email: [email protected] >>> <mailto:[email protected]> >>> <mailto:[email protected] >>> <mailto:[email protected]>> >>> > >>> > Website: www.h3biomedicine.com >>> <http://www.h3biomedicine.com/> >>> <http://www.h3biomedicine.com/> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
