Yes It requires electroporation and very careful handling of the bacmid. Other than that it is a fairly simple process.
Here is an example reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067234/ Artem On Sat, Jul 18, 2020, 9:12 PM Digant Nayak <[email protected]> wrote: > Dear all, > > Sorry for the off topic question, but i assure you that the long term > output of the project has structural biology application. I want to > transform a bacmid into E.coli cell and I was intrigued to find that there > is no protocol for this available on the internet (i would really > appreciate it if somebody proves me wrong and directs me to a link). Is it > similar to transforming a plasmid in E.coli? I would really like to hear > your experiences on this topic. > > Thanks, > Digant > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
