Hi Digant, As Artem mentioned, it's a fairly simple process and it's similar to plasmid transformation in E. coli. We work extensively with insect cells in our lab, though we have never used electroporation, the normal chemical transformation works quite well. Although bacmid requires careful handling and you will need to isolate it using ethanol/isopropanol precipitation owing to its size (no usage of columns).
Also, for transformation of your plasmid, you require E. coli cells which contain bacmid transposition machinery (e.g. DH10Bac cells). More information on Bac-to-Bac system you can find in this guide from invitrogen- http://tools.thermofisher.com/content/sfs/manuals/bactobac_man.pdf Best, Archit On Sun, Jul 19, 2020 at 3:12 AM Digant Nayak <[email protected]> wrote: > Dear all, > > Sorry for the off topic question, but i assure you that the long term > output of the project has structural biology application. I want to > transform a bacmid into E.coli cell and I was intrigued to find that there > is no protocol for this available on the internet (i would really > appreciate it if somebody proves me wrong and directs me to a link). Is it > similar to transforming a plasmid in E.coli? I would really like to hear > your experiences on this topic. > > Thanks, > Digant > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
