Dear Rafal, The difference map you show in your attachment has an echo of the main structure sort of look. So, I suggest you examine your diffraction images specifically looking out for a supercell of weak intensity spots, not picked up by the autoindexing, between the main lattice of intensities, which provided your current unit cell. Best wishes, John Emeritus Professor John R Helliwell DSc
> On 29 Jul 2020, at 13:42, Rafal Dolot <[email protected]> wrote: > > Dear CCP4 users, > > I'm working on the another nucleic acid duplex structure. After several > stages of rebuilding and refinement I observed something like a "shadow" of > the second duplex position. Have you any ideas to explain this phenomena? > Should I place the second molecule with occupancy completed to the first one? > Or maybe is it a kind of disorder in crystal lattice? Data were collected to > 1.02A using synchrotron radiation (processed with XDS), but I have another > datasets collected to lower resolution (from Zn-SAD) with visible similar > effects. Refinement was done using Refmac (R/Rfree 21.5/24.0). Data not > appeared to be twinned, but Xtriage detected pseudo-translational symmetry. > > Thank you for any advices. > > Rafal > > |----------------------------------------------| > |Rafal Dolot, Ph.D. | > | | > |Polish Academy of Sciences | > |Centre of Molecular and Macromolecular Studies| > |Department of Bioorganic Chemistry | > |Macromolecular Crystallography Team | > |Sienkiewicza 112 | > |90-363 Lodz, Poland | > |Phone: +48(42)6803215 | > |Cell: +48 502897781 | > |----------------------------------------------| > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ > <duplex.png> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
