Is the R factor for the best solution really > 40% - if so there must be some major errors to correct. Eleanor
On Fri, 31 Jul 2020 at 11:08, John R Helliwell <jrhelliw...@gmail.com> wrote: > Dear Rafal, > The difference map you show in your attachment has an echo of the main > structure sort of look. So, I suggest you examine your diffraction images > specifically looking out for a supercell of weak intensity spots, not > picked up by the autoindexing, between the main lattice of intensities, > which provided your current unit cell. > Best wishes, > John > Emeritus Professor John R Helliwell DSc > > > > > > On 29 Jul 2020, at 13:42, Rafal Dolot <rdo...@cbmm.lodz.pl> wrote: > > > > Dear CCP4 users, > > > > I'm working on the another nucleic acid duplex structure. After several > stages of rebuilding and refinement I observed something like a "shadow" of > the second duplex position. Have you any ideas to explain this phenomena? > Should I place the second molecule with occupancy completed to the first > one? Or maybe is it a kind of disorder in crystal lattice? Data were > collected to 1.02A using synchrotron radiation (processed with XDS), but I > have another datasets collected to lower resolution (from Zn-SAD) with > visible similar effects. Refinement was done using Refmac (R/Rfree > 21.5/24.0). Data not appeared to be twinned, but Xtriage detected > pseudo-translational symmetry. > > > > Thank you for any advices. > > > > Rafal > > > > |----------------------------------------------| > > |Rafal Dolot, Ph.D. | > > | | > > |Polish Academy of Sciences | > > |Centre of Molecular and Macromolecular Studies| > > |Department of Bioorganic Chemistry | > > |Macromolecular Crystallography Team | > > |Sienkiewicza 112 | > > |90-363 Lodz, Poland | > > |Phone: +48(42)6803215 | > > |Cell: +48 502897781 | > > |----------------------------------------------| > > > > ######################################################################## > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > > <duplex.png> > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
