Hi Guohui, Are you sure this is caused by disulfide bond formation? Do you have any reducing agent in your buffer? 5 Cys residues in 30 amino acids looks very much like a zinc finger to me. Is there any evidence for that? Are there any other zinc binding residues, i.e. His in this region?
Good luck, Bernhard From: CCP4 bulletin board <[email protected]> on behalf of Guohui SHANG <[email protected]> Reply to: Guohui SHANG <[email protected]> Date: Tuesday, 15 September 2020 at 4:22 pm To: "[email protected]" <[email protected]> Subject: [ccp4bb] A Problem of my Research Protein Hi Everyone， Well,My Research Protein is easily Dimerzation caused by Disulfide bond for many Cys.And it leads to the SEC peak too wide about 5 mL. Then I Cut N-ter 30 Amino Acids in which 5 spaced Cys,but the SEC Peak is still wide and hard to Crystallize（as the protein is very pure and clean）.Besides, I have tried different Buffer pH ＜ pI or pH ＞ pI，but the SEC peak Do not Work at all. Anyone could offer your kindly ideas,I would thank you very much！ ________________________________ 发自我的iPhone ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 _______________________________________________ The information in this email is confidential and intended solely for the addressee. You must not disclose, forward, print or use it without the permission of the sender. The Walter and Eliza Hall Institute acknowledges the Wurundjeri people of the Kulin Nation as the traditional owners of the land where our campuses are located and the continuing connection to country and community. _______________________________________________ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
